P-015. ICSI with immotile spermatozoa: validity of the hypo-osmotic swelling test in the selection of viable spermatozoa

The use of a modified hypo-osmotic swelling test for the selection of viable ejaculated and testicular immotile spermatozoa in ICSI H.N.Sallam1,2,3, A.Farrag2, A.F.Agameya1,2, F.Ezzeldin2, A.Eid2 and A.Sallam1,2

A modified hypo-osmotic solution was used to select viable ejaculated and testicular spermatozoa to perform intracytoplasmic sperm injection (ICSI) in 27 treatment cycles from patients with total absence of sperm motility. The treatment cycles consisted of 15 cycles in which ejaculated spermatozoa were used and 12 cycles in which testicular spermatozoa were used. The hypo-osmotic solution consi...

Evaluating the Functional Status of Spermatozoa in Smokers Using Hypo-osmotic Swelling Test

evaluating the functional status of spermatozoa in smokers using hypo-osmotic swelling test

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Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hypo-osmotic solutions.

In order to find an optimal hypo-osmotic swelling test (HOST) and to identify viable sperm cells from patients with asthenozoospermia for intracytoplasmic sperm injection (ICSI), we tested single motile and non-motile spermatozoa in four hypo-osmotic solutions by micromanipulation. The four solutions were: A, H2O; B, 50 mOsm NaCl; C, 150 mOsm NaCl and D, 150 mOsm sodium citrate and fructose. Eo...

Outcome of ICSI Cycles using Pentoxifylline or Theophylline to Identify Viable Spermatozoa in Patients with all Immotile Spermatozoa

Sperm motility is an important parameter for success in intracytoplasmic sperm injection (ICSI) treatment. The sperm motility is due to its tail. If the sperm is not moving, it could be dead or alive. Normal live births have been obtained after ICSI with immotile sperm [1,2]. Various procedures have been applied to pick viable sperm for ICSI including, hypo-osmotic swelling (HOS) test [3], addi...